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Image Search Results


Table of Materials

Journal: Journal of visualized experiments : JoVE

Article Title: Assessment and characterization of hyaloid vessels in mice

doi: 10.3791/59222

Figure Lengend Snippet: Table of Materials

Article Snippet: Apply one drop of pupil-dilating drug solution (see ) per eye to dilate mouse pupils immediately following anesthesia. table ft1 table-wrap mode="anchored" t5 caption a7 Name of Material/ Equipment Company Catalog Number Comments/Description AK-Fluor® (fluorescein injection, USP) Akorn 17478-253-10 Anti-CD31 antibody Abcam ab28364 Antifade mounting medium Thermo Fisher S2828 Antifade Mounting Medium with DAPI Vector Laboratories H-1200 Artificial tear eyedrop Systane N/A Bovine serum albumin (BSA) Sigma-Aldrich A2058 C57BL/6J mice The Jackson Laboratory Stock NO: 000664 Calcium chloride (CaCl2) Sigma-Aldrich C1016 Cryostat Leica CM3050S Cryostat Leica CM3050 S Cyclopentolate hydrochloride and phenylephrine hydrochloride eyedrop Cyclomydril N/A Gelatin Sigma-Aldrich G9382 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 ThermoFisher Scientific A-11008 Heating board Lab-Line Instruments Inc. N/A Isolectin GS-IB4, 594 conjugate ThermoFisher Scientific I21413 Ketamine hydrochloride injection KetaVed NDC 50989-996-06 Lrp5 −/− mice The Jackson Laboratory Stock NO. 005823 Developed by Deltagen Inc., San Mateo, CA Micron IV and OCT Phoenix Research Labs N/A Imaging software: InSight Microscope Zeiss discovery v8 Microsurgery forceps Scanlan International 4004–05 Microsurgery scissors Scanlan International 6006–44 Optimal cutting temperature compound Tissue-Tek 4583 Optimal cutting temperature compound Agar Scientific AGR1180 Paraformaldehyde (16%) Electron Microscopy Sciences 15710 Peel-A-Way disposable embedding molds (tissue molds) Fisher Scientific 12–20 Phosphate-buffered saline (PBS) buffer (10X) Teknova P0496 Slide cover glass Premiere 94-2222-10 Superfrost microscope slides Fisherbrand 12-550-15 Triton X-100 Sigma-Aldrich X100 Xylazine sterile solution Akorn: AnaSed NDC: 59399-110-20 Open in a separate window 1.1.5.

Techniques: Injection, Plasmid Preparation, Imaging, Software, Microscopy, Electron Microscopy, Saline, Sterility